Didactics

Employees of the Department of Biochemistry and Microbiology in the following subjects: biochemistry, general biochemistry of food, biochemistry with elements of biophysics, plant biochemistry, microbiology, bioinformatics, conduct classes with full-time and part-time students of the following Faculties: Biology and Biotechnology, Agriculture and Ecology, Breeding, Bioengineering and Conservation Animal, Horticulture, Human Nutrition, Food Technology, and Civil and Environmental Engineering. In addition to the basic biochemistry and microbiology course, there are many other basic and elective courses.

In addition, the Department conducts classes in genetics and molecular biology of prokaryotes and molecular biology, which are aimed at providing students with practical knowledge in the field of genetic manipulation in accordance with the latest knowledge in this field. The topics of the exercises are selected to cover a logical thematic and experimental sequence from gene cloning from the source organism to obtaining a transgenic organism. Participants of the exercises have a chance to gain laboratory skills and a holistic view of the issues of biotechnology, with particular emphasis on their use in plant biotechnology.

Courses conducted by the Department of Biochemistry and Microbiology
- Basic of Plant Biochemistry and Physiology
- The anaerobic life of bacteria
- Biocenosis of freshwater systems
- Biochemistry
- Ecological biochemistry
- Experimental biochemistry
- General and food biochemistry
- Plant biochemistry
- Animal biochemistry
- Bioinformatics
- Molecular biology
- Biology of selected groups of microorganisms
- Enzymology
- Enzymology and biochemical techniques
- Evolutionary aspects of symbiotic nitrogen fixation
- Prokaryote physiology
- Genetics and molecular biology of prokaryotes
- Informatics
- How microorganisms give form to the world
- Genome analysis methodology
- Microbiology of soil and plants
- Microbiology
- Soil microbiology
- General microbiology
- Industrial microbiology
- Agricultural microbiology
- Environmental microbiology
- Microorganisms in agriculture
- A new research approach and a revolution in microbiology
- Fundamentals of mycology
- Fundamentals of environmental mycology
- Problems and methods of modern microbiological diagnostics
- Industrial use of microorganisms
- Regulation of metabolism
- Environmental and technological microbiological processes
- Information technology
- Introduction to bioinformatics
- Selected techniques of proteomic analyzes

Exercise materials

For exercises in enzymology for students of the first and second degree studies in all fields of study, we recommend “Praktikum z enzymologii”, 2nd edition, revised edited by dr hab. Jolanta M. Dzik. SGGW Publishing House 2017.

Isolating invertase

Basics of plant biochemistry and physiology

Topics of diploma theses

List of proposed topics for theses to be performed at the Department of Biochemistry and Microbiology

Engineering work (agriculture and ecological engineering)

Bachelor's thesis (biology)

Promoter	Topic
dr hab. Tomasz Stępkowski	Application of the DnaK gene in metagenomics of the Rhizobiales community
dr hab. Tomasz Stępkowski	Molecular and phylogenetic characteristics of rhizobium strains isolated from the nodules of Fabaceae plants growing in areas contaminated with heavy metals
dr hab. Małgorzata Dudkiewicz	Methods of modeling protein structures – comparison of publicly available servers and tools
dr hab. inż. Urszula Jankiewicz	Biosurfactants – structure, synthesis regulation mechanisms, application
dr hab. inż. Urszula Jankiewicz	Microbial lipases – general characteristics, molecular mechanisms of synthesis regulation, importance
dr hab. inż. Urszula Jankiewicz	Mushroom chitinases – general characteristics, molecular mechanisms of synthesis regulation, importance
dr hab. inż. Urszula Jankiewicz	Molecular mechanisms of regulation of bacterial chitinase synthesis
dr hab. inż. Urszula Jankiewicz	Application of microbial lacases in the degradation of xenobiotics
dr hab. inż. Urszula Jankiewicz	The mechanism of keratin degradation by enzymes of bacterial origin
dr hab. inż. Urszula Jankiewicz	Interactions of plants and bacteria synthesizing ACC deaminase
dr hab. inż. Urszula Jankiewicz	Detection of quorum sensing particles synthesized by environmental bacteria
dr hab. inż. Urszula Jankiewicz	Molecular identification and metabolic/enzymatic characteristics of bacteria isolated from old heaps and mines in the Tatra National Park
dr hab. Edyta Zdunek-Zastocka	The role of proline in the plant response to stress factors
dr hab. Edyta Zdunek-Zastocka	Mechanism of action and metabolism of selected pesticides
dr hab. Edyta Zdunek-Zastocka	Transgenic plants in the study of abscisic acid metabolism and function
dr inż. Agnieszka Grabowska	Edible plant vaccines
dr inż. Agnieszka Grabowska	The use of mutants in the functional analysis of genes
dr Małgorzata Grzesiuk-Bieniek	The influence of pharmaceuticals on freshwater organisms
dr inż. Małgorzata Nykiel	Plant polyphenols and their role in response to stress
dr inż. Małgorzata Nykiel	Proteolytic and proteasomal protein degradation in water deficit conditions
dr inż. Małgorzata Nykiel	The importance of carbonylation as one of the ways of protein modification under the influence of drought
dr inż. Małgorzata Nykiel	Regulation of the tuberization process in potatoes
dr inż. Sławomir Orzechowski	Effect of drought on the activity of aminotransferases in Solanum tuberosum
dr inż. Beata Prabucka	The role of two-domain cystatins in the regulation of the activity of cysteine endopeptidases
dr Hanna Rekosz-Burlaga	Assessment of the influence of IAA-producing bacteria on selected oat growth parameters in laboratory conditions (research work)
dr Hanna Rekosz-Burlaga	Searching for effective biocontrol factors in the protection of BIO citrus fruits during their storage (research work)
dr Hanna Rekosz-Burlaga	Evaluation of the antagonistic activity of bacteria and yeasts against selected plant pathogens (research work)
dr Hanna Rekosz-Burlaga	Antibiotic resistance of soil microorganisms (theoretical work)
dr Hanna Rekosz-Burlaga	Application potential of selected mushrooms (theoretical work)
dr Joanna Szewińska	The role of caspases and calpains and their endogenous inhibitors in plant stress response

Master's thesis (biology)

Promoter	Topic
dr hab. Tomasz Stępkowski	Application of nitrogenase coding genes in metagenomic studies of the Rhizobiales community
dr hab. Tomasz Stępkowski	Molecular and phylogenetic characteristics of rhizobium strains isolated from Genisteae and Loteae nodules growing in areas with alkaline soils
dr hab. inż. Urszula Jankiewicz	Molecular characteristics of fungal lacases
dr hab. inż. Urszula Jankiewicz	Purification and biochemical characterization of Yarrowia lipolytica yeast extracellular hydrolases
dr hab. inż. Urszula Jankiewicz	Isolation and identification of fungi synthesizing extracellular laccases. Identification of genes coding the tested laccases
dr hab. inż. Urszula Jankiewicz	Participation of chitinases in inhibiting the growth of fungal phytopathogens. Characteristics of the studied chitinases
dr hab. inż. Urszula Jankiewicz	Lipases of halophilic microorganisms – biochemical/molecular characteristics
dr hab. Edyta Zdunek-Zastocka	Does overproduction of proline in transgenic Arabidopsis plants increase their stress tolerance?
dr hab. Edyta Zdunek-Zastocka	Does overexpression of the abscisic acid metabolism genes increase the stress tolerance of Arabidopsisplants?
dr hab. Edyta Zdunek-Zastocka	Preparation and purification of recombinant abscisic acid glucosyltransferase protein in Pichia pastoris cells
dr inż. Agnieszka Grabowska	Functional analysis of the At3g03910 gene – identification of Arabidopsis thaliana mutants
dr inż. Agnieszka Grabowska	Optimization of glutamate dehydrogenase protein expression in E.colicells
dr inż. Agnieszka Grabowska	Molecular analysis of insertional mutants of glutamate dehydrogenase in radish
dr Małgorzata Grzesiuk-Bieniek	The influence of pharmaceuticals on freshwater organisms
dr inż. Małgorzata Nykiel	Plant polyphenols and their role in response to stress
dr inż. Małgorzata Nykiel	Change of the proteome in potato roots in conditions of water deficit
dr inż. Sławomir Orzechowski	Effect of drought on the activity of D-enzyme inSolanum tuberosum
dr inż. Beata Prabucka	Comparison of the usefulness of various methods of extraction and pretreatment of endopeptidases based on native electrophoretic analysis
dr inż. Beata Prabucka	Molecular characteristics of gliadin hydrolysing endopeptidases in the first day of triticale kernel germination

Master's thesis (agriculture and ecological engineering)

Back

Didactics

Courses conducted by the Department of Biochemistry and Microbiology

Exercise materials

Report templates

Consultation hours

Open laboratories

Health and Safety

Exercises in biochemistry

1 – Properties of amino acids and proteins

2 – Quantative protein determination

3 – Vitamin Contents determination

4 – Factors affecting enzyme activity

5 – Nitrate

6 – Amylase

7 – Desalting the protein solution

8 – AlaAT

9 – Proteolytic enzymes

10 – Lipase

11 – Nitrate reductase

Exercises in enzymology

1 – B-fructofuranosidase

2 – Denaturating electrophoresis

3 – Enzymatic inhibition

4 – GDH

5 – Immobilization on chitosan

6 – Native electrophoresis

7 – Phytic acid and inorganic phosphorus

8 – Purification of invertase from yeast

9 – Renin_Rennet

Basics of plant biochemistry and physiology

Topics of diploma theses

Engineering work (agriculture and ecological engineering)

Bachelor's thesis (biology)

Master's thesis (biology)

Master's thesis (agriculture and ecological engineering)