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Didactics

Employees of the Department of Biochemistry and Microbiology in the following subjects: biochemistry, general biochemistry of food, biochemistry with elements of biophysics, plant biochemistry, microbiology, bioinformatics, conduct classes with full-time and part-time students of the following Faculties: Biology and Biotechnology, Agriculture and Ecology, Breeding, Bioengineering and Conservation Animal, Horticulture, Human Nutrition, Food Technology, and Civil and Environmental Engineering. In addition to the basic biochemistry and microbiology course, there are many other basic and elective courses.

In addition, the Department conducts classes in genetics and molecular biology of prokaryotes and molecular biology, which are aimed at providing students with practical knowledge in the field of genetic manipulation in accordance with the latest knowledge in this field. The topics of the exercises are selected to cover a logical thematic and experimental sequence from gene cloning from the source organism to obtaining a transgenic organism. Participants of the exercises have a chance to gain laboratory skills and a holistic view of the issues of biotechnology, with particular emphasis on their use in plant biotechnology.

  • Courses conducted by the Department of Biochemistry and Microbiology

    • Basic of Plant Biochemistry and Physiology
    • The anaerobic life of bacteria
    • Biocenosis of freshwater systems
    • Biochemistry
    • Ecological biochemistry
    • Experimental biochemistry
    • General and food biochemistry
    • Plant biochemistry
    • Animal biochemistry
    • Bioinformatics
    • Molecular biology
    • Biology of selected groups of microorganisms
    • Enzymology
    • Enzymology and biochemical techniques
    • Evolutionary aspects of symbiotic nitrogen fixation
    • Prokaryote physiology
    • Genetics and molecular biology of prokaryotes
    • Informatics
    • How microorganisms give form to the world
    • Genome analysis methodology
    • Microbiology of soil and plants
    • Microbiology
    • Soil microbiology
    • General microbiology
    • Industrial microbiology
    • Agricultural microbiology
    • Environmental microbiology
    • Microorganisms in agriculture
    • A new research approach and a revolution in microbiology
    • Fundamentals of mycology
    • Fundamentals of environmental mycology
    • Problems and methods of modern microbiological diagnostics
    • Industrial use of microorganisms
    • Regulation of metabolism
    • Environmental and technological microbiological processes
    • Information technology
    • Introduction to bioinformatics
    • Selected techniques of proteomic analyzes
Exercise materials

For exercises in enzymology for students of the first and second degree studies in all fields of study, we recommend “Praktikum z enzymologii”, 2nd edition, revised edited by dr hab. Jolanta M. Dzik. SGGW Publishing House 2017.

Isolating invertase

Report templates

Quantitative report

Quality report

Consultation hours

consultation hours for employees of the Department of Biochemistry and Microbiology winter semester 2019/2020

Open laboratories

Instructions

Health and Safety

  • Basics of plant biochemistry and physiology

Topics of diploma theses

List of proposed topics for theses to be performed at the Department of Biochemistry and Microbiology

  • Engineering work (agriculture and ecological engineering)

    Promoter Topic
    dr hab. inż. Urszula Jankiewicz Participation of fungal/bacterial lacases in the biodegradation of xenobiotics
    dr hab. inż. Urszula Jankiewicz Mechanisms of bacterial resistance to heavy metals and/or xenobiotics
    dr hab. inż. Urszula Jankiewicz Synthesis of biosurfactants by bacteria isolated from polluted environments
    dr hab. inż. Urszula Jankiewicz Plant chitinases – functions, synthesis regulation mechanism
    dr inż. Agnieszka Grabowska The use of biotechnological methods in plant breeding
    dr inż. Agnieszka Grabowska Transgenic plants and their role in agriculture
    dr inż. Małgorzata Nykiel Polyphenols – truth and myths

  • Bachelor's thesis (biology)

    Promoter Topic
    dr hab. Tomasz Stępkowski Application of the DnaK gene in metagenomics of the Rhizobiales community
    dr hab. Tomasz Stępkowski Molecular and phylogenetic characteristics of rhizobium strains isolated from the nodules of Fabaceae plants growing in areas contaminated with heavy metals
    dr hab. Małgorzata Dudkiewicz Methods of modeling protein structures – comparison of publicly available servers and tools
    dr hab. inż. Urszula Jankiewicz Biosurfactants – structure, synthesis regulation mechanisms, application
    dr hab. inż. Urszula Jankiewicz Microbial lipases – general characteristics, molecular mechanisms of synthesis regulation, importance
    dr hab. inż. Urszula Jankiewicz Mushroom chitinases – general characteristics, molecular mechanisms of synthesis regulation, importance
    dr hab. inż. Urszula Jankiewicz Molecular mechanisms of regulation of bacterial chitinase synthesis
    dr hab. inż. Urszula Jankiewicz Application of microbial lacases in the degradation of xenobiotics
    dr hab. inż. Urszula Jankiewicz The mechanism of keratin degradation by enzymes of bacterial origin
    dr hab. inż. Urszula Jankiewicz Interactions of plants and bacteria synthesizing ACC deaminase
    dr hab. inż. Urszula Jankiewicz Detection of quorum sensing particles synthesized by environmental bacteria
    dr hab. inż. Urszula Jankiewicz Molecular identification and metabolic/enzymatic characteristics of bacteria isolated from old heaps and mines in the Tatra National Park
    dr hab. Edyta Zdunek-Zastocka The role of proline in the plant response to stress factors
    dr hab. Edyta Zdunek-Zastocka Mechanism of action and metabolism of selected pesticides
    dr hab. Edyta Zdunek-Zastocka Transgenic plants in the study of abscisic acid metabolism and function
    dr inż. Agnieszka Grabowska Edible plant vaccines
    dr inż. Agnieszka Grabowska The use of mutants in the functional analysis of genes
    dr Małgorzata Grzesiuk-Bieniek The influence of pharmaceuticals on freshwater organisms
    dr inż. Małgorzata Nykiel Plant polyphenols and their role in response to stress
    dr inż. Małgorzata Nykiel Proteolytic and proteasomal protein degradation in water deficit conditions
    dr inż. Małgorzata Nykiel The importance of carbonylation as one of the ways of protein modification under the influence of drought
    dr inż. Małgorzata Nykiel Regulation of the tuberization process in potatoes
    dr inż. Sławomir Orzechowski Effect of drought on the activity of aminotransferases in Solanum tuberosum
    dr inż. Beata Prabucka The role of two-domain cystatins in the regulation of the activity of cysteine endopeptidases
    dr Hanna Rekosz-Burlaga Assessment of the influence of IAA-producing bacteria on selected oat growth parameters in laboratory conditions (research work)
    dr Hanna Rekosz-Burlaga Searching for effective biocontrol factors in the protection of BIO citrus fruits during their storage (research work)
    dr Hanna Rekosz-Burlaga Evaluation of the antagonistic activity of bacteria and yeasts against selected plant pathogens (research work)
    dr Hanna Rekosz-Burlaga Antibiotic resistance of soil microorganisms (theoretical work)
    dr Hanna Rekosz-Burlaga Application potential of selected mushrooms (theoretical work)
    dr Joanna Szewińska The role of caspases and calpains and their endogenous inhibitors in plant stress response

  • Master's thesis (biology)

    Promoter Topic
    dr hab. Tomasz Stępkowski Application of nitrogenase coding genes in metagenomic studies of the Rhizobiales community
    dr hab. Tomasz Stępkowski Molecular and phylogenetic characteristics of rhizobium strains isolated from Genisteae and Loteae nodules growing in areas with alkaline soils
    dr hab. inż. Urszula Jankiewicz Molecular characteristics of fungal lacases
    dr hab. inż. Urszula Jankiewicz Purification and biochemical characterization of Yarrowia lipolytica yeast extracellular hydrolases
    dr hab. inż. Urszula Jankiewicz Isolation and identification of fungi synthesizing extracellular laccases. Identification of genes coding the tested laccases
    dr hab. inż. Urszula Jankiewicz Participation of chitinases in inhibiting the growth of fungal phytopathogens. Characteristics of the studied chitinases
    dr hab. inż. Urszula Jankiewicz Lipases of halophilic microorganisms – biochemical/molecular characteristics
    dr hab. Edyta Zdunek-Zastocka Does overproduction of proline in transgenic Arabidopsis plants increase their stress tolerance?
    dr hab. Edyta Zdunek-Zastocka Does overexpression of the abscisic acid metabolism genes increase the stress tolerance of Arabidopsisplants?
    dr hab. Edyta Zdunek-Zastocka Preparation and purification of recombinant abscisic acid glucosyltransferase protein in Pichia pastoris cells
    dr inż. Agnieszka Grabowska Functional analysis of the At3g03910 gene – identification of Arabidopsis thaliana mutants
    dr inż. Agnieszka Grabowska Optimization of glutamate dehydrogenase protein expression in E.colicells
    dr inż. Agnieszka Grabowska Molecular analysis of insertional mutants of glutamate dehydrogenase in radish
    dr Małgorzata Grzesiuk-Bieniek The influence of pharmaceuticals on freshwater organisms
    dr inż. Małgorzata Nykiel Plant polyphenols and their role in response to stress
    dr inż. Małgorzata Nykiel Change of the proteome in potato roots in conditions of water deficit
    dr inż. Sławomir Orzechowski Effect of drought on the activity of D-enzyme inSolanum tuberosum
    dr inż. Beata Prabucka Comparison of the usefulness of various methods of extraction and pretreatment of endopeptidases based on native electrophoretic analysis
    dr inż. Beata Prabucka Molecular characteristics of gliadin hydrolysing endopeptidases in the first day of triticale kernel germination

  • Master's thesis (agriculture and ecological engineering)

    Promoter Topic
    dr hab. inż. Urszula Jankiewicz Isolation of bacteria antagonistic to phytopathogenic fungi
    dr hab. inż. Urszula Jankiewicz Degradation of aniline and its derivatives by aerobic soil microorganisms
    dr hab. inż. Urszula Jankiewicz The share of yeasts in biological plant protection
    dr inż. Małgorzata Nykiel Protein degradation mechanisms
    dr inż. Sławomir Orzechowski Analysis of the effect of drought on the activity of amylases in potato leaves
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