Practicum for exercises

Department of Plant Physiology

Anna_Dzierzynska@sggw.pl

Table of Contents

The subject of plant ecophysiology

Description of activities in the subject
Syllabus
Exercise program in the subject
Principles of passing the course Ecophysiology of plants

Introduction to exercises

ABC of laboratory equipment Preparation of solutions
Reports and reports

Tasks to be performed

The task of Plant growth in aquatic cultures Task Characteristics of artificial light sources Task Determination of chlorophyll content Task Determination of the relative content of chlorophyll and flavonoids Task Measure Fn dependency on PAR Task Measure Fn dependency on[CO2] Task Measure breathing intensity with an infrared gas analyzer Task Measure the intensity of breathing with oxygen electrode Task Marking RWC and WSD leaves Task Measurement of the transpiration intensity using the weight method Task Measurement of water potential using the pressure chamber method

Germination tasks

Seed sterilization methods Task 1 2015 Influence of water potential on swelling, germination and initial growth of seedlings 2015 Task 2 2015 Effect of oxygen on respiration and initial growth of seedlings 2015 Task 3 2015 Influence of temperature on the rate of swelling of seeds and seedling growth Task 4 The effect of light on the seeding of seeds of different species Task 5 2015 The effect of metal ion contamination on seedling germination and growth Task 6 2015 Effect of allelopathic compounds in the environment on seed germination and seedling growth Task 7 Influence of the pH of the medium on the germination and growth of seedlings and changes in the pH of the seedlings Task 8 2015 The effect of selected phytohormones on the germination and growth of seedlings

Performing measurements with instruments and apparatuses

Instructions for the use of automatic pipettes Performing the measurement with the LI 1800 spectroradiometer Performed the measurement with the light meter Li 190 with Li 185B Performing the measurement with the SPECOL spectrophotometer Performing the measurement with the GL Spectrolux spectrometer Performing the measurement with the SPAD 502 meter Performing the measurement with the Dualex4 meter Performing the measurement with the R and FR light meters Measurement with a CO2 analyzer, room 091 Measurement with an infrared CO2 analyzer, room 089 Performing the measurement of the oxygen electrode Measurement of the pressure chamber Performing the measurement with the FMS2 fluorimeter Measurement with a Li 3000A leaf surface meter Performing the measurement with the meter of the relative chlorophyll content CL 01 Performing the measurement with the HR 33T dew point microvoltometer

Principles of apparatus and instruments operation

The principle of spectroradiometer measurement Principle of PAR intensity measurement with Li meter The principle of measurement with a spectrophotometerMeasurement principle with the SPAD 502 meter Measurement principle with the Dualex meter 4 Measurement principle with R and FR light meters Principle of Fn and Rd measurement with an infrared gas analyzer Measurement principle of the oxygen electrode Measurement principle: PMS pressure chamber Principle of chlorophyll fluorescence measurement with a fluorimeter The principle of measurement with the Wescor HR 33T psychrometer Principles of leaf area measurement and LAI index

Introduction to exercises

Practicum-radiation and introduction dyes Praktikum – water management introduction